THE IMPACT OF LOCAL PRP INJECTION ON THE GASTRIC MUCOSA LIPID PEROXIDATION IN RATS WITH PEPTIC ULCERS AND HEMORRHAGIC SHOCK
EAES Academy. Petrushenko V. 07/05/22; 363173; P218
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Abstract
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The aim of the study was to evaluate changes in gastric mucosa Malondialdehyde (MDA) after local PRP injection in rats with peptic ulcers and hemorrhagic shock in experiment.
Methods. The study was performed on 91 Wistar rats (average weight of animals was 183±16 grams) according to local and international rules for working with experimental animals. We randomly divide all animals in 5 groups: Control Group (n=7) – intact animals; Comparison Group (n=21) – gastric ulcer; Group 1 (n=21) – gastric ulcer + hemorrhagic shock; Group 2 (n=21) – gastric ulcer + hemorrhagic shock + local injection of 0.1 ml of 0,9% sodium chloride; Group 3 (n=21) – gastric ulcer + hemorrhagic shock + local injection of 0.1 ml platelet-rich plasma (PRP).
Peptic ulcers were modeled using our modification of type 2 acetic acid ulcer model (Susumu Okabe, 2005). Hemorrhagic shock was modeled by 3–3.5 ml blood sampling. On 1st, 7th and 14th day measurement of mucosal MDA levels were performed.
Results:
In all groups and on all control days of the study, MDA activity levels were higher than in the control group.
On day 1, MDA activity in Groups 1, 2, 3 were significantly higher (p<0.05) than in the Comparison Group. Moreover, the indices in the Groups 1, 2, 3 didn’t significantly differ from each other (p>0.05).
On day 7 of the study, we didn’t reveal a significant difference in the level of the studied indicator between the Comparison Group and Group 3 (p<0.05), as well as between Groups 1 and 2 (p>0.05). Moreover, in pairwise statistical comparison, the indicators in the Comparison Group and Group 3 were statistically significantly lower than the similar levels in Groups 1 and 2 (p<0.05).
On the day 14, there were no differences between MDA levels in Comparison Group and Groups 1 and 2. In the same time MDA levels in Group 3 were significantly lower than levels in all other groups (p<0.05) and approached the indices of the control group.
Conclusions. Hemorrhagic shock is accompanied by an intensification of lipid peroxidation in the gastric mucosa. Local PRP injection in rats with peptic ulcers and hemorrhagic shock allows to inhibit lipid peroxidation in the periulcelar zone.
Methods. The study was performed on 91 Wistar rats (average weight of animals was 183±16 grams) according to local and international rules for working with experimental animals. We randomly divide all animals in 5 groups: Control Group (n=7) – intact animals; Comparison Group (n=21) – gastric ulcer; Group 1 (n=21) – gastric ulcer + hemorrhagic shock; Group 2 (n=21) – gastric ulcer + hemorrhagic shock + local injection of 0.1 ml of 0,9% sodium chloride; Group 3 (n=21) – gastric ulcer + hemorrhagic shock + local injection of 0.1 ml platelet-rich plasma (PRP).
Peptic ulcers were modeled using our modification of type 2 acetic acid ulcer model (Susumu Okabe, 2005). Hemorrhagic shock was modeled by 3–3.5 ml blood sampling. On 1st, 7th and 14th day measurement of mucosal MDA levels were performed.
Results:
In all groups and on all control days of the study, MDA activity levels were higher than in the control group.
On day 1, MDA activity in Groups 1, 2, 3 were significantly higher (p<0.05) than in the Comparison Group. Moreover, the indices in the Groups 1, 2, 3 didn’t significantly differ from each other (p>0.05).
On day 7 of the study, we didn’t reveal a significant difference in the level of the studied indicator between the Comparison Group and Group 3 (p<0.05), as well as between Groups 1 and 2 (p>0.05). Moreover, in pairwise statistical comparison, the indicators in the Comparison Group and Group 3 were statistically significantly lower than the similar levels in Groups 1 and 2 (p<0.05).
On the day 14, there were no differences between MDA levels in Comparison Group and Groups 1 and 2. In the same time MDA levels in Group 3 were significantly lower than levels in all other groups (p<0.05) and approached the indices of the control group.
Conclusions. Hemorrhagic shock is accompanied by an intensification of lipid peroxidation in the gastric mucosa. Local PRP injection in rats with peptic ulcers and hemorrhagic shock allows to inhibit lipid peroxidation in the periulcelar zone.
The aim of the study was to evaluate changes in gastric mucosa Malondialdehyde (MDA) after local PRP injection in rats with peptic ulcers and hemorrhagic shock in experiment.
Methods. The study was performed on 91 Wistar rats (average weight of animals was 183±16 grams) according to local and international rules for working with experimental animals. We randomly divide all animals in 5 groups: Control Group (n=7) – intact animals; Comparison Group (n=21) – gastric ulcer; Group 1 (n=21) – gastric ulcer + hemorrhagic shock; Group 2 (n=21) – gastric ulcer + hemorrhagic shock + local injection of 0.1 ml of 0,9% sodium chloride; Group 3 (n=21) – gastric ulcer + hemorrhagic shock + local injection of 0.1 ml platelet-rich plasma (PRP).
Peptic ulcers were modeled using our modification of type 2 acetic acid ulcer model (Susumu Okabe, 2005). Hemorrhagic shock was modeled by 3–3.5 ml blood sampling. On 1st, 7th and 14th day measurement of mucosal MDA levels were performed.
Results:
In all groups and on all control days of the study, MDA activity levels were higher than in the control group.
On day 1, MDA activity in Groups 1, 2, 3 were significantly higher (p<0.05) than in the Comparison Group. Moreover, the indices in the Groups 1, 2, 3 didn’t significantly differ from each other (p>0.05).
On day 7 of the study, we didn’t reveal a significant difference in the level of the studied indicator between the Comparison Group and Group 3 (p<0.05), as well as between Groups 1 and 2 (p>0.05). Moreover, in pairwise statistical comparison, the indicators in the Comparison Group and Group 3 were statistically significantly lower than the similar levels in Groups 1 and 2 (p<0.05).
On the day 14, there were no differences between MDA levels in Comparison Group and Groups 1 and 2. In the same time MDA levels in Group 3 were significantly lower than levels in all other groups (p<0.05) and approached the indices of the control group.
Conclusions. Hemorrhagic shock is accompanied by an intensification of lipid peroxidation in the gastric mucosa. Local PRP injection in rats with peptic ulcers and hemorrhagic shock allows to inhibit lipid peroxidation in the periulcelar zone.
Methods. The study was performed on 91 Wistar rats (average weight of animals was 183±16 grams) according to local and international rules for working with experimental animals. We randomly divide all animals in 5 groups: Control Group (n=7) – intact animals; Comparison Group (n=21) – gastric ulcer; Group 1 (n=21) – gastric ulcer + hemorrhagic shock; Group 2 (n=21) – gastric ulcer + hemorrhagic shock + local injection of 0.1 ml of 0,9% sodium chloride; Group 3 (n=21) – gastric ulcer + hemorrhagic shock + local injection of 0.1 ml platelet-rich plasma (PRP).
Peptic ulcers were modeled using our modification of type 2 acetic acid ulcer model (Susumu Okabe, 2005). Hemorrhagic shock was modeled by 3–3.5 ml blood sampling. On 1st, 7th and 14th day measurement of mucosal MDA levels were performed.
Results:
In all groups and on all control days of the study, MDA activity levels were higher than in the control group.
On day 1, MDA activity in Groups 1, 2, 3 were significantly higher (p<0.05) than in the Comparison Group. Moreover, the indices in the Groups 1, 2, 3 didn’t significantly differ from each other (p>0.05).
On day 7 of the study, we didn’t reveal a significant difference in the level of the studied indicator between the Comparison Group and Group 3 (p<0.05), as well as between Groups 1 and 2 (p>0.05). Moreover, in pairwise statistical comparison, the indicators in the Comparison Group and Group 3 were statistically significantly lower than the similar levels in Groups 1 and 2 (p<0.05).
On the day 14, there were no differences between MDA levels in Comparison Group and Groups 1 and 2. In the same time MDA levels in Group 3 were significantly lower than levels in all other groups (p<0.05) and approached the indices of the control group.
Conclusions. Hemorrhagic shock is accompanied by an intensification of lipid peroxidation in the gastric mucosa. Local PRP injection in rats with peptic ulcers and hemorrhagic shock allows to inhibit lipid peroxidation in the periulcelar zone.
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